COVID-19 Vaccines: All Transfection Methods will use (Luciferase Gene)
- Moderator
- Nov 7, 2020
- 1 min read
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
Lipid Nanoparticles for Gene Delivery
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
Electroporation for nanomedicine: a review
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
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